Phosphodiesterase 9 (PDE9) regulates bovine tracheal smooth muscle relaxation

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Phosphodiesterase 9 (PDE9) regulates bovine tracheal smooth muscle relaxation

The present study was designed to clarify phosphodiesterase 9 (PDE9) expression in bovine tracheal smooth muscle tissue, and to elucidate that PDE9 may contribute to the regulation of airway relaxation. PDE9 mRNA expression was detected in bovine tracheal smooth muscle. Sodium nitroprusside (an NO donor) and BAY 73-6691 (a selective PDE9 inhibitor) reduced high K+- and carbachol-induced contrac...

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The role of myosin phosphorylation in regulating smooth muscle contraction has been investigated by quantitating the myosin phosphate content of tracheal smooth muscle frozen during contraction or relaxation. Myosin was purified from quick-frozen muscle samples with the aid of antibodies prepared against tracheal smooth muscle myosin, and the phosphate content was determined after separation of...

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ژورنال

عنوان ژورنال: Journal of Veterinary Medical Science

سال: 2018

ISSN: 0916-7250,1347-7439

DOI: 10.1292/jvms.18-0011